Pervasive regulation of Drosophila Notch target genes by GY-box-, Brd-box-, and K-box-class microRNAs

Regulation of Notch Signaling by an Evolutionary Conserved DEAD Box RNA Helicase, Maheshvara in Drosophila melanogaster.

Notch signaling is an evolutionary conserved process that influences cell fate determination, cell proliferation, and cell death in a context-dependent manner. Notch signaling is fine-tuned at multiple levels and misregulation of Notch has been implicated in a variety of human diseases. We have characterized maheshvara (mahe), a novel gene in Drosophila melanogaster that encodes a putative DEAD...

In vivo transcriptional regulation of N-Myc target genes is controlled by E-box methylation.

N-Myc is a transcription factor that forms heterodimers with the protein Max and binds gene promoters by recognizing a DNA sequence, CACGTG, called E-box. The identification of N-myc target genes is an important step for understanding N-Myc biological functions in both physiological and pathological contexts. In this study, we describe the identification of N-Myc-responsive genes through chroma...

Black Box , State Box , and Clear Box Concepts

M a y / J u n e 2 0 0 0 I E E E S O F T W A R E 71 This was the situation in a company I worked for some years ago: people were whispering in the corners, discussing explanations and scapegoats—half-hearted attempts to put out the fire. We were rapidly losing money and self-respect, until one manager stepped forward and admitted: “This piece of software is fatal for us. We must completely rebui...

Identification and Distinct Regulation of Yeast TATA Box-Containing Genes

Despite being one of the first eukaryotic transcriptional regulatory elements identified, the sequence of a native TATA box and its significance remain elusive. Applying criteria associated with TATA boxes we queried several Saccharomyces genomes and arrived at the consensus TATA(A/T)A(A/T)(A/G). Approximately 20% of yeast genes contain a TATA box. Strikingly, TATA box-containing genes are asso...

Analysis in Bla k - Box